Pure-IP™ Western Blot Detection test kit
Size
20 blots
Catalog no.
PRB-5002
Price
434 EUR
This reagent is purified
0.98
Molecular weight
kiloDalton
Conjugation and form
host specific
We send this reagant on
Room temperature
Use of this test kit or product
Molecular biology
Antigen
the sequence of the protein
Type of reagent
Cell Signaling and Protein Biology
Species compatible to
human cells and cell lines and other cells
Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,19
The temperatureerature for storage is
Store the HRP conjugate at -20°C. Store the remaining components at 4°C.
Related techniques
Cell Signaling and Protein Biology, Protein Analysis Tools, IP Western Blot Detection
Test
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
Use of the reagent
Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection test kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.
Description
The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.The ultrapure Pure-IP™ Blot test kit supplied from Cellular biology labs is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.