Donkey anti-Rabbit IgG (H&L) - Affinity Pure, FITC Conjugate, min x w/mouse IgG or serum proteins


Size

2 mg

Catalog no.

MBS687585

Price

299 EUR

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Group

Sera

Tissue

serum

Conjugation

Anti-FITC Antibody

Additional isotype

Heavy & Light chains,IgG

Other size

please contact us to order other different size

Latin name

Equus africanus asinus,Mus musculus,Oryctolagus cuniculus

Test

A high affinity purification column was use to purify anti-Rabbit IgG (H&L) - Pure, FITC Conjugate, min x w/mouse IgG serum proteins by MyBioSource by chromatographic size exclusion.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Properties

This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The ultrapure anti-Rabbit IgG (H&L) - Pure, FITC Conjugate, min x w/mouse IgG serum proteins supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Serum should be stored at +5°C