Goat anti-Human IgG (H&L) - Affinity Pure, FITC Conjugate, min x w/rat IgG[IgG (H&L)]


Size

NA

Catalog no.

MBS686200

Price

5 EUR

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Conjugation

Anti-FITC Antibody

Products_type

Secondary Antibody

Additional isotype

Heavy & Light chains,IgG

Latin name

Capra aegagrus hircus,Rattus norvegicus

Reactivity

Human.Cross Reactivity: Based on IEP, no reactivity is observed to:non-immunoglobulin human serum immunoglobulinsrat IgG

Test

A high affinity purification column was use to purify Goat anti- IgG (H&L) - Pure, FITC Conjugate, min x w/ IgG[IgG (H&L)] by MyBioSource by chromatographic size exclusion.MyBioSource supplies antibodies that are for research of human proteins.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The ultrapure Goat anti- IgG (H&L) - Pure, FITC Conjugate, min x w/ IgG[IgG (H&L)] supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Properties

This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.