The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,19
The temperatureerature for storage is
Store the HRP conjugate at -20°C. Store the remaining components at 4°C.
Cell Signaling and Protein Biology, Protein Analysis Tools, IP Western Blot Detection
SDS or safty data sheet
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
Use of the reagent
Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection test kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.
The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.The ultrapure Pure-IP™ Blot test kit supplied from Cellular biology labs is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.